Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
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چکیده
منابع مشابه
Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...
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ژورنال
عنوان ژورنال: DNA Research
سال: 2008
ISSN: 1340-2838,1756-1663
DOI: 10.1093/dnares/dsm023